DNA ligases seal five′-PO4 and 3′-OH polynucleotide ends by using a few nucleotidyl transfer methods involving ligase-adenylate and DNA-adenylate intermediates. DNA ligases are critical guardians of genomic integrity, and ligase dysfunction underlies human genetic illness syndromes. Crystal constructions of DNA ligases certain to nucleotide and nucleic acid substrates have illuminated how ligase reaction chemistry is catalyzed, how ligases figure out weakened DNA finishes, and how protein domain movements and Lively-site reworking are used to choreograph the tip-joining pathway.
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